歡迎選擇我們，上海恒遠(yuǎn)生物科技有限公司！免費(fèi)服務(wù)熱線：400-0899-208 防偽查詢

語(yǔ)言：Eng

您當(dāng)前的位置：: 首頁(yè)>; 產(chǎn)品中心>; 抗體>; 單克隆抗體>; PKM Monoclonal Antib...

單克隆抗體

PKM Monoclonal Antibody

　　恒遠(yuǎn)生物抗體平臺(tái)現(xiàn)已擁有4萬(wàn)多種抗體(含單抗和多抗)，包括人類常用抗體，模式生物類相關(guān)抗體、小分子抗體、二抗、標(biāo)簽抗體及內(nèi)參抗體等。并以每年4000種的速度在持續(xù)增長(zhǎng)。

　　恒遠(yuǎn)生物擁有專業(yè)的質(zhì)控團(tuán)隊(duì)，配備先進(jìn)實(shí)驗(yàn)儀器，現(xiàn)已成功地建立了ChIP平臺(tái)，因此我們的抗體不僅能提供Biotin、FITC、HRP等標(biāo)記，大部分抗體都能進(jìn)行ELISA、WB、IHC、IF、IP、ChIP等應(yīng)用驗(yàn)證。

　　我們致力于為所有用戶提供高親和力、高特異性的優(yōu)質(zhì)抗體。

產(chǎn)品詳情相關(guān)產(chǎn)品

PKM Monoclonal Antibody

貨號(hào)：

HYK-200023
規(guī)格：
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate
All lanes: PKM antibody at 1:4000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 1min

Western Blot
Positive WB detected in: Mouse Heart tissue, Mouse Brain tissue, Mouse Skeletal Muscle tissue
All lanes: PKM antibody at 1:4000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa

Western Blot
Positive WB detected in: Rat Heart tissue, Rat Spleen tissue, Rat Brain tissue
All lanes: PKM antibody at 1:4000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 55-60 kDa
Observed band size: 55-60 kDa

Western Blot
Positive WB detected in: MCF-7 whole cell lysate at 40μg, 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg
All lanes: PKM antibody at 1:4000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 5min

IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

產(chǎn)品詳情

產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) PKM Monoclonal antibody
Uniprot No.：

P14618
基因名：

PKM
別名：

CTHBP antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; OIP-3 antibody; Opa-interacting protein 3 antibody; p58 antibody; pkm antibody; PKM1 antibody; PKM2 antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase muscle isozyme antibody; Pyruvate kinase PKM antibody; THBP1 antibody; Thyroid hormone-binding protein 1 antibody; Tumor M2-PK antibody
宿主：

Mouse
反應(yīng)種屬：

Human, Rat, Mouse, Rabbit
免疫原：

Recombinant Human Pyruvate kinase PKM protein (2-531AA)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG1
純化方式：

>95%, Protein G purified
克隆號(hào)：

6C3C7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, FC, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:4000-1:256000
IHC	1:200-1:500
IF	1:150-1:300
FC	1:50-1:200
IP	1μl-4μl

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.