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6*His Monoclonal Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

6*His Monoclonal Antibody

貨號：

HYK-200066
規(guī)格：
圖片：
Western Blot
Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa

Western Blot
Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa

產(chǎn)品詳情

產(chǎn)品描述：

6*His monoclonal antibody CSB-MA000011M0m was produced in mouse immunized by using HHHHHH(6x His) synthetic peptide conjugate to KLH as the immunogen. The immunogen, 6x His, a short peptide, consists of six histidine residues (His-His-His-His-His-His). It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
This 6*His Monoclonal Antibody was tested in the WB, IHC, ICC and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.
別名：

6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
宿主：

Mouse
反應(yīng)種屬：

N/A
免疫原：

HHHHHH(6x His) synthetic peptide conjugate to KLH
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2a
純化方式：

>95%, Protein A purified
克隆號：

3G5H8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.