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BCL2 Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

BCL2 Antibody

貨號：

HYK-200087
規(guī)格：
圖片：
Western Blot
Positive WB detected in:HepG2 whole cell lysate,Jurkat whole cell lysate,MCF-7 whole cell lysate
All lanes:BCL2 antibody at 1μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 KDa
Observed band size: 26 KDa

IHC image of CSB-RA002611A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

產(chǎn)品詳情

產(chǎn)品描述：

BCL2 monoclonal antibody CSB-RA002611A0HU was produced in the rabbit immunized by using the synthesized peptide as the immunogen. The target protein BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. This protein can inhibit caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Diseases associated with BCL2 include High Grade B-Cell Lymphoma With Myc And/ Or Bcl2 And/Or Bcl6 Rearrangement and Follicular Lymphoma 1.
This BCL2 monoclonal antibody was tested in the ELISA, WB, IHC and FC applications. The non-conjugated IgG got purified by antigen affinity. It reacts with the BCL2 proteins of human or mouse or rat-origin and may be used to detect the endogenous levels of BCL2 protein.
Uniprot No.：

P10415
基因名：

BCL2
別名：

Apoptosis regulator Bcl 2 antibody; Apoptosis regulator Bcl-2 antibody; Apoptosis regulator Bcl2 antibody; AW986256 antibody; B cell CLL/lymphoma 2 antibody; B cell leukemia/lymphoma 2 antibody; Bcl-2 antibody; Bcl2 antibody; BCL2_HUMAN antibody; C430015F12Rik antibody; D630044D05Rik antibody; D830018M01Rik antibody; Leukemia/lymphoma; B-cell; 2 antibody; Oncogene B-cell leukemia 2 antibody; PPP1R50 antibody; Protein phosphatase 1 regulatory subunit 50 antibody; Protein phosphatase 1; regulatory subunit 50 antibody
反應(yīng)種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.